MindMap Gallery DNA Technology Tree Map
Using recombinant DNA technology and synthetic DNA, literally, any DNA sequence may be created and introduced in this mind map.
Mind maps are a great resource to help you study. A mind map can take complex topics like plant kingdom and illustrate them into simple points, as shown above.
Mind maps are useful in constructing strategies. They provide the flexibility of being creative, along with the structure of a plan.
Vitamins and minerals are essential elements of a well-balanced meal plan. They help in ensuring that the body is properly nourished. A mind map can be used to map out the different vitamins a person requires.
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DNA-Technology-Concept-Map
Explain rDNA
- Cutting DNA and pasting DNA fromdifferent sources (also known as DNAcloning)
- Involves several important tools
- Restriction enzymes
- Cloning vector
- DNA ligase
Cloning vs Gene Cloning
- Clone (Greek): described a cutting that isused to copy a plant.
- Orchid
- Roseses
- Tapioca
- A state that all organisms share exact samegenetic material.
- Cloning
- Reproduction of organism as a wholewithout sexual fertilization between maleand female gametes.
- Gene cloning
- Insertion of foreign DNA into anotherorganism.
What are required?
- Restriction endonuclease
- Also called DNA cutting enzyme orrestriction endonuclease (RE)
- The act of cutting called digestion
- Primarily in bacteria (as defense againstbacteriophage infection.
- Known as restriction site
- Other types of RE produce DNA fragmentswith double stranded ends known as bluntends
- An enzyme responsible of joining two DNAfragments
- Synthesize phosphodiester bond
- Two DNA fragments
- Between two compatible sticky ends or twoblunt ends.
- Common features between RE and DNAligase
- DNA cloning vector
- Pieces of DNA that can accept, carry andreplicate (clone) other pieces of DNA
- Important features
- Has the ability to replicate autonomously inbacterial host
- Has a site for insertion of foreign DNA
- Has a selectable antibiotic resistantmarker(s)
- Ampicilin
- Kanamycin
- Tetracyclin
- DNA plasmid
- Screening of recombinants
- Usage of selectable markers available onDNA plasmid
- Antibiotic resistant genes
- Lac Z gene (blue/white colony screening)
- Recombinant DNA tools