se peptone (meat and casein) :3 gm Lactose monohydrate :10 gm Bile salts :1.5 gm Sodium chloride : 5 gm Neutral red : 0.03 gm Crystal Violet :0.001 gm Peptone (Pancreatic digest of gelatin) : 17 gm ProteoAgar :13.5 gmH

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1.MacConkey agar is used for the isolation of gram-negative enteric bacteria and the differentiation of lactose fermenting from lactose non-fermenting gram-negative bacteria.

2. Pancreatic digest of gelatin and peptones (meat and casein) provide the essential nutrients, vitamins and nitrogenous factors required for growth of microorganisms.

3.Lactose monohydrate is the fermentable source of carbohydrate. The selective action of this medium is attributed to crystal violet and bile salts, which are inhibitory to most species of gram-positive bacteria.

4. Sodium chloride maintains the osmotic balance in the medium. Neutral red is a pH indicator that turns red at a pH below 6.8 and is colorless at any pH greater than 6.8. Agar is the solidifying agent.

MacConkey agar is used for the isolation of gram-negative enteric bacteria.It is used in the differentiation of lactose fermenting from lactose non-fermenting gram-negative bacteria.It is used for the isolation of coliforms and intestinal pathogens in water, dairy products and biological specimens.opic