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MindMap Gallery Detection method of autophagy activity in cell lines
Detection method of autophagy activity in cell lines
Methods for detecting autophagy activity in cell lines mainly include biochemical assays, flow cytometry, and reporter assays. Biochemical assays assess autophagy activity by measuring the LC3-II/I ratio and p62 protein degradation. Flow cytometry utilizes fluorescently labeled antibodies against autophagy-related proteins for quantitative cellular analysis. Reporter assays utilize GFP-LC3 and mCherry-LC3 fusion protein systems to monitor autophagy flux in real-time.
Edited at 2024-12-24 22:45:02
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