Neutrophil alkaline phosphatase staining

acid phosphatase staining

specific esterase staining

non-specific esterase staining

azo coupling reaction

periodic acid Schiff reaction

Prussian blue reaction

Myeloperoxidase (MPO)—a protein most abundant in neutrophils

Grain system

monocytic system

other cells

MPO staining is the first choice and the most important cytochemical staining method to identify acute leukemia cell types.

1) Acute myeloid leukemia

2) Acute promyelocytic leukemia

3) Acute myeloid-monocytic leukemia

4) Acute monocytic leukemia

5) Acute lymphoblastic leukemia

6) Pure erythroleukemia

1) Grain system

2) Red series

3) Lymphatic system

4) Monocyte system

5) Megakaryon system

1) Identification of erythroid diseases

2) Identification of acute leukemia cell types

3) Identification of other cells

NAP mainly exists in the cytoplasm of mature neutrophils, so the cytoplasm of mature neutrophils is positive, and other cells are negative.

NAP is a marker enzyme of neutrophils and mainly exists in mature neutrophils.

NAP activity reflects the maturity and function of mature granulocytes. As the cells mature, the activity of the enzyme gradually increases. When neutrophils are activated, the positive rate and score of NAP increase.

Chronic myeloid leukemia (chronic phase)

acute purulent infection

acute myeloid leukemia

aplastic anemia