Select an appropriate strain of plague bacteria, culture and amplify it to obtain a sufficient amount of vaccine raw materials.

Control the culture conditions and provide appropriate nutrients, temperature and pH value to promote the growth and reproduction of strains.

For the preparation of freeze-dried live plague vaccines, plague bacteria usually need to be treated, such as through heat treatment or chemical treatment, to make them lose their replication ability and pathogenicity.

The treated plague bacteria are still immunogenic but do not cause disease, thus ensuring the safety of the vaccine.

The treated Y. pestis bacteria are freeze-dried through appropriate methods to maintain their immunogenicity and stability.

During the freeze-drying process, parameters such as temperature and humidity need to be controlled to ensure that the vaccine quality meets the requirements.

Freeze-dried live plague vaccine should be stored under low temperature, dry, and protected light conditions to prevent it from losing immune activity.

Vaccines usually need to be stored in a freezer or freezer, keeping the temperature below -20 degrees Celsius.

During transportation, appropriate measures need to be taken to ensure that the temperature and humidity of the vaccine are controlled to avoid the vaccine losing its immunological activity during transit.

During transportation, attention must also be paid to protecting the vaccine from external shocks and vibrations to ensure the integrity and stability of the vaccine.

Freeze-dried live plague vaccine is usually administered by subcutaneous injection, which effectively stimulates the body's immune system to produce an immune response.

When inoculating, you need to pay attention to the reasonable dosage and injection site, and follow the relevant regulations and operating guidelines of the medical and health institutions.

Safety evaluation of freeze-dried live plague vaccine is an important step to ensure its safety.

Safety evaluation usually includes vaccine virulence testing, sensitization testing, mutagenicity testing, etc. to ensure that the vaccine will not cause serious side effects and risks during application.