MindMap Gallery Medicine - General Stool Examination
This is a mind map about general examination of stool, including physical examination, chemical examination, microscopic examination, etc. Hope this helps!
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This is a mind map about bacteria, and its main contents include: overview, morphology, types, structure, reproduction, distribution, application, and expansion. The summary is comprehensive and meticulous, suitable as review materials.
This is a mind map about plant asexual reproduction, and its main contents include: concept, spore reproduction, vegetative reproduction, tissue culture, and buds. The summary is comprehensive and meticulous, suitable as review materials.
This is a mind map about the reproductive development of animals, and its main contents include: insects, frogs, birds, sexual reproduction, and asexual reproduction. The summary is comprehensive and meticulous, suitable as review materials.
General stool examination
Purpose and significance
① Understand whether there is infection, bleeding, malignant tumor, etc. in the digestive tract.
②Understand the functional status of the gastrointestinal tract and hepatobiliary system based on the characteristics and composition of feces.
③Check intestinal pathogenic bacteria to assist in the diagnosis of intestinal infectious diseases.
④Fecal occult blood test can be used as a screening test for malignant tumors of the digestive tract.
⑤Based on the color of feces and the examination of fecal bile hormone, it is helpful to identify the type of jaundice.
Physical examination
quantity
Healthy adults: 100-300g
Appearance: Normal adult stool is soft and tangible
Character changes and clinical significance:
color
The color of healthy adult feces is yellow-brown due to the presence of stercobilin. Infant feces are mostly yellow-green or golden because biliverdin has not been converted into bilirubin.
Stool color and possible causes:
Odor: After food is acted upon by intestinal bacteria, it produces indole, hydrogen sulfide and other odorous substances.
Parasites: Intestinal parasites and their eggs can be excreted in the feces. The adult worms are sometimes visible to the naked eye when they are excreted in the feces. The eggs often require microscopic examination. (Hookworm eggs can only be found after fecal screening)
Stones: stones passed in the feces, most commonly gallstones.
chemical examination
fecal occult blood test
Definition: The amount of upper gastrointestinal bleeding is less than 5ml, there is no visible blood in the stool, and the red blood cells are destroyed, and no red blood cells are seen under the microscope. Bleeding that requires chemical methods, immunological methods, etc. to be confirmed is called occult blood, and the test to detect fecal occult blood is called fecal occult blood test (FOBT).
Detection methods and principles:
1) Chemical method: It is a commonly used method. Commonly used methods include o-toluidine method (high sensitivity, high false positives), guaiac method (low sensitivity, high specificity), etc. The traditionally used chemical methods have been replaced by the current test strip method (unstable, specificity). lower).
immunological methods
The specificity and sensitivity are equal to/better than the guaiac method and are highly specific.
Reference interval: negative
Clinical significance: 1) Judgment of gastrointestinal bleeding; 2) Differentiation of peptic ulcer and tumor bleeding
fecal fat test
Purpose: One of the projects to help understand the digestion and absorption functions of the digestive tract.
Inspection methods and principles:
Reference interval: 1) Total fat content in adult feces (calculated as total fatty acids): 2 ~ 5g/24h, or 7.3% ~ 27.6% of dry feces; 2) Adults eat 50 ~ 150g/24h fat, and the amount of fat excreted is <7g, Fat absorption rate > 95%.
Clinical significance: When the ability to digest and absorb fat decreases, the total fat content in the feces increases significantly. If the total fat content in the feces exceeds 6g in 24 hours, it is called steatorrhea. Increased fecal fat can be seen in: ① Pancreatic diseases: chronic pancreatitis, pancreatic cancer, etc. ②Hepatobiliary diseases: cholestatic jaundice, insufficient bile secretion, viral hepatitis, cirrhosis, etc. ③Small intestinal lesions: celiac disease, Whipple's disease, protein-losing gastrointestinal disease, etc. ④Others: gastric and duodenal fistula, peptic ulcer, etc.
fecal bile pigment determination
Fecal bile pigments include fecal bilirubin, fecal bilinogen, and fecal bilin.
The bilirubin in normal human bile is decomposed into fecal bile by bacteria in the terminal ileum and colon. Except for part of it being reabsorbed into the human enterohepatic circulation, most of it is oxidized into fecal bile in the colon, making the feces brown. , and excreted with feces.
Qualitative detection of fecal bile pigments can also be performed, but it is not commonly used in clinical practice.
microscopy
cell
white blood cells (pus cells)
The common white blood cells in feces are neutrophils, and those with intact morphology are no different from granulocytes in blood.
Under pathological conditions, neutrophils are gray-white, have swollen cell bodies, are necrotic, have incomplete broken structures, are filled with small particles in the cytoplasm, and have unclear nuclei (ie, pus cells), and often appear in piles.
There are no/occasionally white blood cells in the feces of healthy people.
Under pathological conditions, the number of white blood cells is related to the degree and location of inflammation. ①Inflammation of the small intestine: The increase in white blood cells is not obvious, usually less than 15/HP, scattered and difficult to identify. ② Bacillary dysentery and ulcerative colitis: A large number of white blood cells and small phagocytes that engulf foreign bodies appear, concentrated in areas with mucus or pus and blood in the feces. ③Irritable bowel syndrome and intestinal parasitic diseases (especially hookworm disease and amoebic dysentery): More eosinophils are seen, often accompanied by Charcot-Leyden crystals.
large phagocytes
There are no large phagocytes (macrophages) in normal stool
It is common in acute bacillary dysentery, also seen in acute hemorrhagic enteritis, and occasionally in ulcerative colitis.
Bacillary dysentery: More phagocytes are common.
Epithelial Cells
Most of the epithelial cells that are exfoliated in a small amount under physiological conditions are destroyed and are difficult to detect in normal stool.
1) In intestinal inflammation, such as cholera, paracholera, necrotizing enteritis, etc., epithelial cells increase; 2) In pseudomembranous colitis, a larger number of intestinal mucosal columnar epithelial cells can be seen in the mucosal mass of the feces, mostly with white blood cells. Co-exist.
tumor cells
Bloody stool smears from patients with sigmoid colon cancer and rectal cancer show piles of cancer cells.
red blood cells
Red blood cells The red blood cells in the feces are grass-green, slightly refractive disc-shaped, and sometimes appear shrunken due to the influence of the pH of the feces.
There are no red blood cells in the feces of healthy people.
Pathogenic microorganisms
parasite eggs
Ascaris eggs, whipworm eggs, hookworm eggs, paragonimiasis eggs, liver fluke eggs, ginger worm eggs, schistosomiasis eggs, etc. are commonly found in fecal smears.
protozoan
1) Entamoeba histolytica: Fresh red mucus is present in the stool during amebic dysentery. Trophozoites or cysts can be seen under the microscope.
2) Giardia lamblia: Mainly found in the thin stools of infected children with diarrhea and tourists.
3) Cryptosporidium: Tiny in size, it can often cause immune deficiency syndrome and diarrhea in children.
bacteria
There are more normal flora in the feces of healthy people. Escherichia coli and Enterococcus are the main ones in the feces of adults. Bacterium dipyrum, Enterobacter, and Enterococcus are the main ones in the feces of young children, accounting for about 1/3 of the dry weight of the feces. Under normal circumstances, an ecological balance is maintained between bacteria and the host, and the ratio of cocci/bacilli in feces is approximately 1:10.
Fungus
Very rare in normal stool.
Fungal spores are 3 to 5 pm in diameter, round or oval, have strong refractive properties, are Gram stain positive, and mostly have hyphae.
Common fungi in feces are common yeast-like fungi and Candida albicans-like fungi.
Excluding contamination of stool specimens, it is common in the stool of patients who take large amounts of antibiotics, hormones, and immunosuppressants.
food debris and crystals
food residue
Fat
Neutral fat (fat droplets): various sizes, round, highly refractive globules, which appear red after Sudan Il staining
Free fatty acids: in the form of flakes or needle bundles, which melt after heating. The flakes turn orange after Sudan dyeing, while the needle bundles are not colored.
Combined fatty acid: It is an insoluble substance formed by the combination of fatty acid with calcium, magnesium, etc. It is yellow, irregular block or flake-shaped, does not dissolve when heated, and is not stained by Sudan River.
starch granules
Rarely seen in normal feces.
muscle fiber
It is light yellow strips and sheets with fine horizontal stripes. It can be dyed red if eosin is added.
Plant cells and plant fibers
In the shape of spiral tubules or honeycombs, plant cells of various shapes can be seen. It is round, oblong, polygonal, with a double-layered cell wall, and there are chlorophyll bodies in the cells. Care must be taken to distinguish them from parasite eggs.
crystallization
A variety of crystals can be seen in the feces, which are generally of no clinical significance.
The occurrence of Charcot-Leyden crystals and heme crystals indicates gastrointestinal bleeding.
Mainly seen in gastrointestinal bleeding, amoebic dysentery, hookworm disease and allergic colitis, which are often accompanied by eosinophilia.
Stool testing is the most direct and reliable way to diagnose intestinal parasitic infections.
[Reference interval] Normal stool microscopic examination shows no red blood cells, occasional white blood cells, no parasites and eggs, and a small amount of food residues.