MindMap Gallery Biochemical testing of proteins
Mind map of biochemical testing of proteins. The detection methods include Kjeldahl method, biuret method, phenol reagent method, ultraviolet absorption method, turbidimetric method, and dye binding method.
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This is a mind map about bacteria, and its main contents include: overview, morphology, types, structure, reproduction, distribution, application, and expansion. The summary is comprehensive and meticulous, suitable as review materials.
This is a mind map about plant asexual reproduction, and its main contents include: concept, spore reproduction, vegetative reproduction, tissue culture, and buds. The summary is comprehensive and meticulous, suitable as review materials.
This is a mind map about the reproductive development of animals, and its main contents include: insects, frogs, birds, sexual reproduction, and asexual reproduction. The summary is comprehensive and meticulous, suitable as review materials.
Biochemical testing of proteins
Overview
Classification of plasma egg quality
Classified by separation method
Salting out method
Albumin/globulin
Electrophoresis
cellulose acetate thin film electrophoresis
agarose gel electrophoresis
polyacrylamide gel electrophoresis
SDS polyacrylamide gel isoelectric two-dimensional electrophoresis
Classified by function
Transportation carrier class
Complement proteins
Immunoglobulins
coagulation proteins
protease inhibitor
protein hormones
acute phase response protein
Physiological functions of plasma protein
Nutritional effect, repair tissue protein
Maintain plasma colloid osmotic pressure
As a carrier of hormones, vitamins, lipids, metabolites, ions, drugs, etc.
As part of the blood pH buffering system
Inhibit cathepsin
Detection
Test items
Tp
Alb
PA
AAG
AAT
Cp
f
β2-MG
CRP
HP
AMG
Detection method
Kjeldahl method
Calculate protein concentration based on average protein nitrogen content of 16%
It is a reference method for determination of protein content.
Advantages: good accuracy, high precision, high sensitivity
Disadvantages: complex operation, time-consuming, not suitable for routine clinical measurement
biuret method
Adjacent peptide bonds (-CO-NH-) in proteins can form stable purple complexes with divalent copper ions in alkaline solutions. The depth of the color is proportional to the serum protein content, so it can be used to measure protein content.
It is a routine method for measuring total serum protein.
Advantages: good specificity, accuracy and precision, simple operation, stable color development
Disadvantages: low sensitivity
phenol reagent method
UV absorption method
Turbidimetry
dye binding method