cell culture laboratory

(1) Aseptic technique

(2) Selection and processing of culture materials

(3) Cell culture types and technologies

(4) Cell morphology examination

(5) Detection and elimination of cultured cell contamination

(6) Cell cryopreservation, recovery and transportation

(1) Cell cycle analysis method

(1) MTT method

(2) Mitotic index method

Principles of immune cell isolation

(1) Preparation of peripheral blood mononuclear cell suspension

(2) Isolation and purification of lymphocytes

(3) Separation of T cells and B cells

(4) Isolation of T cell subsets

(5) Isolation of monocytes and macrophages

(6) Isolation of monocytes and macrophages

(1) Trace cytotoxicity test to detect the number of various types of immune cells

(2) Immunofluorescence staining technology to detect the number of various immune cells

(3) Flow cytometry to detect the number of various immune cells

(4) Immunoenzyme staining technology detects the number of various immune cells

(1) Principle of lymphocyte proliferation test

(2) Morphological examination of lymphocyte proliferation

(3) Morphological examination of lymphocyte proliferation by MTT assay

(1) Morphological examination method---Cytotoxicity test of lymphocytes against tumor cells

(1) Characteristics of stem cells

(2) Stem cell classification

(3) History of stem cell research

(4) Source of stem cells

(5) Differentiation potential of stem cells

(6) Differences between embryonic and adult stem cells

①Nuclear transplantation

②Nuclear fusion

(1) Concept of regenerative medicine

(2) Hematopoietic stem cell transplantation

(3) Autologous limbal stem cell transplantation

(4) Application of neural stem cells

(5) Skin stem cells and beauty

(6) Other applications

(7) Relevant laws and regulations

(1) Advantages and disadvantages of cells

(2) How to choose

(3) Research hot spots

(1) Experiment on affecting tumor cell growth (drug sensitivity test)

(2) Tumor cell migration test

(3) Tumor cell invasion test

(4) Establishment of tumor differentiation induction model

(5) Detection of telomerase activity in tumor cells

(6) Detection of tumor cell genes and their expression products

(1) Experiments on drug transport by cells (intestinal absorption)

(2) Constructing a human blood-brain barrier drug screening model

(3) Micronucleus test-toxicological application

Morphology, growth, culture medium color (pH), contamination or not

Trypan blue, MTT, CCK8, colony formation assay