Increased collagen production is associated with the development and progression of breast cancer, and stromal cells are the primary source of collagen deposition. The expression of collagen P4H is significantly upregulated during the occurrence and development of breast cancer, and the increased expression of P4HA is associated with poor prognosis. Interestingly, induction of P4HA1 expression in cancer cells is necessary for breast cancer metastasis

P4HA1 contributes most of the prolyl 4-hydroxylase activity Newly synthesized HIF-1α is rapidly hydroxylated at proline 402 and (Pro564) by the oxygen-dependent dioxygenase (PHD) family Proline hydroxylation induces HIF-1α ubiquitination and degradation, thereby shortening the half-life of HIF-1 protein

Fig1.P4HA1 protein levels are significantly increased in TNBC

Fig. 2 P4HA1 induces the HIF-1 pathway in breast cancer cells

Fig. 3 P4HA1 enhances HIF-1α protein stability

Fig. 4 P4HA1 enhances HIF-1α stability by modulating α-KG l and succinate levels

P4HA1 and tumors