MindMap Gallery Prokaryotic gene expression regulation
Prokaryotic gene expression regulation is an important research content in the field of biology. It involves how prokaryotes regulate their gene expression according to changes in environmental conditions to adapt to different living environments.
Edited at 2024-11-01 19:54:23This is a mind map about the annual work plan of the three pillars of human resources. The main contents include: strategic human resources planning, talent recruitment and allocation, employee performance management, employee training and development, employee relationships and communication, employee welfare and care, human resources information system construction, regulatory compliance and risk management, and organizational culture construction.
This is a mind map for the diagnosis and treatment of acute cerebral hemorrhage in patients with hemodialysis. The annual incidence of acute cerebral hemorrhage in patients with hemodialysis is (3.0~10.3)/1000, and the main cause is hypertension. Compared with non-dialysis patients, the most common bleeding site is the basal ganglia area, accounting for 50% to 80%; but the bleeding volume is large and the prognosis is poor, and the mortality rate is 27% to 83%. Especially for patients with hematoma >50ml, hematoma enlarged or ventricular hemorrhage on the second day after onset, the prognosis is very poor.
The logic is clear and the content is rich, covering many aspects of the information technology field. Provides a clear framework and guidance for learning and improving information technology capabilities.
This is a mind map about the annual work plan of the three pillars of human resources. The main contents include: strategic human resources planning, talent recruitment and allocation, employee performance management, employee training and development, employee relationships and communication, employee welfare and care, human resources information system construction, regulatory compliance and risk management, and organizational culture construction.
This is a mind map for the diagnosis and treatment of acute cerebral hemorrhage in patients with hemodialysis. The annual incidence of acute cerebral hemorrhage in patients with hemodialysis is (3.0~10.3)/1000, and the main cause is hypertension. Compared with non-dialysis patients, the most common bleeding site is the basal ganglia area, accounting for 50% to 80%; but the bleeding volume is large and the prognosis is poor, and the mortality rate is 27% to 83%. Especially for patients with hematoma >50ml, hematoma enlarged or ventricular hemorrhage on the second day after onset, the prognosis is very poor.
The logic is clear and the content is rich, covering many aspects of the information technology field. Provides a clear framework and guidance for learning and improving information technology capabilities.
Prokaryotic gene expression regulation
Lactose operon and negative control induction system
Other issues in the lac operon
lacA gene and its physiological functions
The acetyltransferase produced by the A gene can acetylate galactoside to avoid degradation and produce harmful derivatives.
Quantitative comparison of lac gene products
In a fully induced cell, the copy number ratio of β⁻galactosidase, permease, and acetyltransferase is 1:0.5:0.2.
Reason: The quantitative differences between different enzymes are due to regulation at the translation level.
Adjustment method
lac mRNA may detach from the ribosome during translation, thereby terminating the translation of the protein chain.
Within the lac mRNA molecule, the A gene is more susceptible to degradation by endonucleases than the Z gene.
Operon fusion and genetic engineering
Coupling of lac operon and pur operon responsible for purine synthesis
The pur operon is "grafted" to the lac promoter to form a fusion gene.
Meaning: Increase protein synthesis.
Tryptophan operon and negative control repression system
Types of specific metabolites regulating activity
Inducible regulation: operon that regulates catabolism, regulated by cAMP-CRP
Repressible regulation: An operon that regulates biosynthesis and is not regulated by cAMP-CRP.
This is the type of trp system
Structure of trp operon
There are 7 structural genes, and the synthesis of trp is divided into 5 steps.
Features
tripR and trpABCDE are not linked
There is a weakener
Promoter and structural genes are separated by leader sequence
trp operon repression system
Supervisor whether transcription starts
Regulatory signal: amount of trp
Weakening effect of trp operon
A 600-fold difference in expression levels of the trp operon was observed at high and low concentrations of tryptophan, whereas repression only reduced transcription 70-fold.