MindMap Gallery Metabolomics analysis methods for cell lines
Metabolomics analysis methods for cell lines encompass metabolite detection and quantification, data processing and analysis, and metabolic pathway analysis. Techniques such as NMR spectroscopy, LC-MS, and GC-MS are utilized to identify and quantify metabolites within cell lines, while advanced methods like stable isotope labeling provide insights into metabolic flux. Data processing involves preprocessing raw data, normalizing metabolite concentrations, and applying statistical methods to identify significant changes, often utilizing bioinformatics tools for pathway mapping and enrichment analysis. Metabolic pathway analysis integrates metabolite data with known metabolic pathways to understand the functional implications of observed changes, including flux balance analysis and metabolic network modeling.
Edited at 2024-12-25 07:11:46