MindMap Gallery Method for detecting the activity of membrane protein complexes in cell lines
The method for detecting the activity of membrane protein complexes involves cell culture and separation, complex purification, activity detection, and data analysis. Firstly, cell lines are cultured under appropriate conditions and membrane fractions are isolated. Subsequently, membrane protein complexes are purified using methods such as affinity chromatography. Then, the activity state of the complexes is detected by enzymatic activity assays, protein-protein interaction analyses, or functional assays. Finally, quantitative analysis of the activity detection results is performed to determine the functional characteristics of the membrane protein complexes.
Edited at 2025-01-12 01:42:41