MindMap Gallery Methods for detecting and eliminating contamination in cell lines
Methods for detecting and eliminating contamination in cell lines include various detection techniques, contamination confirmation, elimination methods, and preventive measures. Initially, potential contamination is identified through microscopic observation of cell morphology changes, bacterial, fungal, and mycoplasma cultures, as well as PCR-based detection of specific pathogens. When signs such as abnormal cell growth or medium discoloration appear, contamination type is confirmed based on detection results. Subsequently, depending on the contamination type, antibiotic or antifungal treatments, or specific mycoplasma eradication agents are applied. In cases of severe contamination, reculturing or introducing uncontaminated cell lines may be considered. To prevent contamination, sterile practices should be enhanced, and the cleanliness of media and experimental equipment should be regularly tested.
Edited at 2025-01-14 12:42:49