MindMap Gallery Proteomic analysis methods for cell lines
This is a mind map about Proteomic analysis methods for cell lines,Main content: Applications,Validation Methods,Bioinformatics Tools,Data Analysis,Mass Spectrometry (MS),Separation Techniques,Sample Preparation.
Edited at 2025-01-20 08:13:29In the introduction section, elastin is a protein that plays a crucial role in the body, endowing tissues with elasticity and toughness. Its properties include high elasticity and good ductility. Elastin has a wide range of sources and is commonly found in animal connective tissues such as skin and blood vessels. When using elastin in products, the amount and method of addition should be determined based on the characteristics of the product. It has many uses and can be added to skincare products to enhance skin elasticity and reduce wrinkles; Improving food texture and enhancing taste in the food industry; In the medical field, it can be used to make elastic scaffolds such as artificial blood vessels, providing support for tissue repair. Reasonable use can play its unique value.
This document aims to guide the proper use of proteins. Proteins are the fundamental substances that make up life and are essential for maintaining normal physiological functions in the body, with a wide variety of types. When using protein, daily diet is an important source, such as meat, eggs, etc., which are rich in high-quality protein. Measuring protein intake can be done using professional tools or methods to ensure that the intake meets the body's needs. When mixing protein powder, it should be prepared according to the recommended ratio, and the water temperature should not be too high to avoid damaging the protein activity. Different groups of people have different protein requirements, such as athletes and fitness enthusiasts who can increase their intake appropriately to meet the needs of body repair and muscle growth.
Introduction to Unsaturated Polyester Resin: It is an important thermosetting resin with various excellent properties. It has a wide range of applications and is used in the construction industry to produce fiberglass products such as doors, windows, decorative panels, etc; Used in the automotive industry for manufacturing body components. The advantages of use include simple molding process, low cost, and chemical corrosion resistance. However, it also faces challenges and limitations, such as relatively poor heat resistance and susceptibility to aging. During use, corresponding protective measures should be taken according to specific application scenarios, such as adding heat-resistant agents, antioxidants, etc., to extend their service life and fully leverage their advantages.
In the introduction section, elastin is a protein that plays a crucial role in the body, endowing tissues with elasticity and toughness. Its properties include high elasticity and good ductility. Elastin has a wide range of sources and is commonly found in animal connective tissues such as skin and blood vessels. When using elastin in products, the amount and method of addition should be determined based on the characteristics of the product. It has many uses and can be added to skincare products to enhance skin elasticity and reduce wrinkles; Improving food texture and enhancing taste in the food industry; In the medical field, it can be used to make elastic scaffolds such as artificial blood vessels, providing support for tissue repair. Reasonable use can play its unique value.
This document aims to guide the proper use of proteins. Proteins are the fundamental substances that make up life and are essential for maintaining normal physiological functions in the body, with a wide variety of types. When using protein, daily diet is an important source, such as meat, eggs, etc., which are rich in high-quality protein. Measuring protein intake can be done using professional tools or methods to ensure that the intake meets the body's needs. When mixing protein powder, it should be prepared according to the recommended ratio, and the water temperature should not be too high to avoid damaging the protein activity. Different groups of people have different protein requirements, such as athletes and fitness enthusiasts who can increase their intake appropriately to meet the needs of body repair and muscle growth.
Introduction to Unsaturated Polyester Resin: It is an important thermosetting resin with various excellent properties. It has a wide range of applications and is used in the construction industry to produce fiberglass products such as doors, windows, decorative panels, etc; Used in the automotive industry for manufacturing body components. The advantages of use include simple molding process, low cost, and chemical corrosion resistance. However, it also faces challenges and limitations, such as relatively poor heat resistance and susceptibility to aging. During use, corresponding protective measures should be taken according to specific application scenarios, such as adding heat-resistant agents, antioxidants, etc., to extend their service life and fully leverage their advantages.
Proteomic analysis methods for cell lines
Sample Preparation
Cell Culture
Growing cells in controlled conditions
Types of media and supplements
Cell Lysis
Physical disruption (sonication, French press)
Chemical lysis (detergents, chaotropes)
Protein Extraction
Solubilization of proteins
Removal of cellular debris
Protein Quantification
Colorimetric assays (Bradford, BCA)
Fluorometric assays
Separation Techniques
1D Electrophoresis
SDS-PAGE
Separation based on size
Staining (Coomassie, silver)
2D Electrophoresis
Isoelectric focusing (IEF)
SDS-PAGE
Separation based on charge and size
Liquid Chromatography (LC)
Reversed-phase chromatography
Ionexchange chromatography
Size-exclusion chromatography
Mass Spectrometry (MS)
Matrix-Assisted Laser Desorption/Ionization (MALDI)
Sample preparation with matrix
Time-of-flight (TOF) analysis
Electrospray Ionization (ESI)
Soft ionization technique
Coupling with LC
Tandem MS (MS/MS)
Fragmentation of ions
Peptide sequencing
Data Analysis
Spectral Analysis
Peak detection
Mass assignment
Database Search
Protein identification
Sequence matching algorithms (Mascot, SEQUEST)
Quantitative Proteomics
Label-free quantification
Isotope labeling (iTRAQ, SILAC)
Bioinformatics Tools
Data Interpretation
Functional annotation
Pathway analysis
Statistical Analysis
Significance testing
Multivariate analysis
Validation Methods
Western Blotting
Antibody-based protein detection
Immunoprecipitation
Isolation of protein complexes
Enzyme-linked Immunosorbent Assay (ELISA)
Quantitative measurement of proteins
Applications
Biomarker Discovery
Identification of disease-related proteins
Drug Target Identification
Characterization of drug-protein interactions
Cell Signaling Studies
Understanding cellular communication networks
Comparative Proteomics
Analyzing differences between cell lines