MindMap Gallery Lipidomics analysis methods for cell lines
This is a mind map about Lipidomics analysis methods for cell lines,Main content: Applications,Bioinformatics tools,Data analysis,Lipid detection,Lipid separation,Sample preparation.
Edited at 2025-01-20 08:30:50In the introduction section, elastin is a protein that plays a crucial role in the body, endowing tissues with elasticity and toughness. Its properties include high elasticity and good ductility. Elastin has a wide range of sources and is commonly found in animal connective tissues such as skin and blood vessels. When using elastin in products, the amount and method of addition should be determined based on the characteristics of the product. It has many uses and can be added to skincare products to enhance skin elasticity and reduce wrinkles; Improving food texture and enhancing taste in the food industry; In the medical field, it can be used to make elastic scaffolds such as artificial blood vessels, providing support for tissue repair. Reasonable use can play its unique value.
This document aims to guide the proper use of proteins. Proteins are the fundamental substances that make up life and are essential for maintaining normal physiological functions in the body, with a wide variety of types. When using protein, daily diet is an important source, such as meat, eggs, etc., which are rich in high-quality protein. Measuring protein intake can be done using professional tools or methods to ensure that the intake meets the body's needs. When mixing protein powder, it should be prepared according to the recommended ratio, and the water temperature should not be too high to avoid damaging the protein activity. Different groups of people have different protein requirements, such as athletes and fitness enthusiasts who can increase their intake appropriately to meet the needs of body repair and muscle growth.
Introduction to Unsaturated Polyester Resin: It is an important thermosetting resin with various excellent properties. It has a wide range of applications and is used in the construction industry to produce fiberglass products such as doors, windows, decorative panels, etc; Used in the automotive industry for manufacturing body components. The advantages of use include simple molding process, low cost, and chemical corrosion resistance. However, it also faces challenges and limitations, such as relatively poor heat resistance and susceptibility to aging. During use, corresponding protective measures should be taken according to specific application scenarios, such as adding heat-resistant agents, antioxidants, etc., to extend their service life and fully leverage their advantages.
In the introduction section, elastin is a protein that plays a crucial role in the body, endowing tissues with elasticity and toughness. Its properties include high elasticity and good ductility. Elastin has a wide range of sources and is commonly found in animal connective tissues such as skin and blood vessels. When using elastin in products, the amount and method of addition should be determined based on the characteristics of the product. It has many uses and can be added to skincare products to enhance skin elasticity and reduce wrinkles; Improving food texture and enhancing taste in the food industry; In the medical field, it can be used to make elastic scaffolds such as artificial blood vessels, providing support for tissue repair. Reasonable use can play its unique value.
This document aims to guide the proper use of proteins. Proteins are the fundamental substances that make up life and are essential for maintaining normal physiological functions in the body, with a wide variety of types. When using protein, daily diet is an important source, such as meat, eggs, etc., which are rich in high-quality protein. Measuring protein intake can be done using professional tools or methods to ensure that the intake meets the body's needs. When mixing protein powder, it should be prepared according to the recommended ratio, and the water temperature should not be too high to avoid damaging the protein activity. Different groups of people have different protein requirements, such as athletes and fitness enthusiasts who can increase their intake appropriately to meet the needs of body repair and muscle growth.
Introduction to Unsaturated Polyester Resin: It is an important thermosetting resin with various excellent properties. It has a wide range of applications and is used in the construction industry to produce fiberglass products such as doors, windows, decorative panels, etc; Used in the automotive industry for manufacturing body components. The advantages of use include simple molding process, low cost, and chemical corrosion resistance. However, it also faces challenges and limitations, such as relatively poor heat resistance and susceptibility to aging. During use, corresponding protective measures should be taken according to specific application scenarios, such as adding heat-resistant agents, antioxidants, etc., to extend their service life and fully leverage their advantages.
Lipidomics analysis methods for cell lines
Sample preparation
Cell lysis
Mechanical disruption
Use of sonication
Use of French press
Chemical lysis
Use of detergents
Use of organic solvents
Protein precipitation
Addition of methanol or acetonitrile
Centrifugation to remove precipitated proteins
Extraction of lipids
Folch method (chloroform/methanol)
Bligh and Dyer method (chloroform/methanol/water)
Lipid separation
Thin-layer chromatography (TLC)
Silica gel plates
Visualization with iodine or specific stains
High-performance liquid chromatography (HPLC)
Normal phase
Separation based on polarity
Reverse phase
Separation based on hydrophobicity
Gas chromatography (GC)
Derivatization of lipids
Formation of volatile derivatives
Separation based on volatility and boiling point
Lipid detection
Mass spectrometry (MS)
Electrospray ionization (ESI)
Soft ionization technique
Matrix-assisted laser desorption/ionization (MALDI)
Use of matrix to assist in ionization
Tandem MS (MS/MS)
Fragmentation of lipids for structural information
Nuclear magnetic resonance (NMR) spectroscopy
Identification of lipid classes and fatty acid composition
Quantification of lipids
Data analysis
Peak identification
Use of databases (e.g., Lipid Maps, HMDB)
Comparison with standards
Quantification
Internal standards
Addition of known amounts of isotopically labeled lipids
External standards
Calibration curves for quantification
Statistical analysis
Multivariate analysis
Principal component analysis (PCA)
Partial least squares discriminant analysis (PLS-DA)
Univariate analysis
ANOVA
ttest
Bioinformatics tools
Lipid identification software
LipidSearch
LipidBlast
Data normalization
Normalization to total lipid content
Normalization to cell number or protein content
Pathway analysis
Integration with genomic and proteomic data
Metabolic pathway mapping
Applications
Disease biomarker discovery
Identification of lipid changes in disease states
Correlation with disease progression
Drug discovery
Assessment of drug effects on lipid metabolism
Identification of potential drug targets
Systems biology
Understanding lipid metabolism in cellular systems
Modeling lipid networks and their interactions