Service content: Provide preliminary preparation and preliminary data processing services to ensure the accuracy and reliability of sequencing results.

Technical breakthrough: Team founder Shi Xianming proposed new gene detection technology and invented related products and services

Products and services: Plant DNA capacity detection kit, animal/plant DNA ploidy detection kit, plant flow cytometry analysis, etc.

Market monopoly: break the monopoly of foreign companies, form industrial strategic alliances, and sell front-end sequencing service business

Policy support: From the 12th Five-Year Plan to the 14th Five-Year Plan, national policies support the development of gene sequencing technology.

Technical barriers: The development of high-end equipment and reagents faces high technical barriers and complex processes.

Market status: The growth rate of the domestic gene sequencing market is higher than that of the global market, but the upstream market is still monopolized overseas.

Product price: The price of plant flow cytometry test kit is 1,000-3,500 yuan per time.

Technological innovation: quick leaf cutting, nuclear lysate, flow cytometry automatic injection tray, etc.

Service advantages: easy to operate, good safety, high sensitivity, high detection efficiency and affordable price.

Breaking the monopoly: Breaking the monopoly of foreign kits through professional technology and services.

Technical support: Provide technical support to small and micro enterprises and private enterprises to fill the gap in domestic plant gene sequencing needs.

Advantages: Independent research and development, publish high-level papers, possess patented technology, easy to operate and affordable price.

Disadvantages: difficult technical operation, expensive equipment, restricted by regulations, and low public acceptance.

Market prospects: The global gene sequencing market is developing rapidly, and the Chinese market is gradually rising.

Market pain points: high technical barriers, difficult operation, and difficult innovation

Solution: Das develops new patented technologies to improve detection efficiency and accuracy and reduce costs.

Marketing methods: point-to-point sales, Internet platform promotion, official website sales.

Customer group: scientific research institutions, biological enterprises, and university laboratories.

Model: Technology Service Product Sales.

Partners: Wuhan University, Northwest Hubei Medicinal Plant Laboratory, Wuhan Fraser, BGI.

Step 1: Establish a company, optimize the product, and complete the iteration of the testing reagent technology within 1 year.

Step 2: Horizontal expansion of business areas and add new histone extraction kits and other businesses within 3 years.

Step 3: Deep genetic sequencing development, becoming a leading front-end service company within 7 years.

Equity structure: The company's registered capital is 1 million yuan, Shi Xianming accounts for 40%, Ma Xueping and Yu Tingting each account for 30%.

Revenue forecast: revenue in the first year is 1 million, 2.5 million in the second year, and exceed 5 million in the third year.

Patent risk: Improve confidentiality agreements and professional legal services.

Talent risk: Relying on universities to gather high-level talents.

Market risk: strong market competition and low product popularity.

Credit risk: kit accuracy issues.

Financial risk: inventory realization risk.

Brand risk: Increase popularity and expand influence.

Genome wave: Since the human genome was successfully deciphered in the late 20th century, life science research has entered the "genome wave" stage.

Policy support: From the 12th Five-Year Plan to the 14th Five-Year Plan, national policies support the development of gene sequencing technology.

Market size: The global gene sequencing market is developing rapidly, and the Chinese market is gradually rising.

Gene detection technology: Obtain genomic information of biological samples through genetic testing equipment and is applied to scientific research or clinical diagnosis.

Gene sequencing technology: The technology to obtain the base arrangement order of specific DNA fragments is the basis of molecular biology research and genetic modification.

Technology development: Gene sequencing technology has promoted the progress of genomics, biomedical research and clinical diagnosis.

Market status: The growth rate of domestic gene sequencers and consumables market is higher than the global average, but the upstream market is still monopolized overseas.

Technical barriers: The development of high-end equipment and reagents faces high technical barriers and complex processes.

Market demand: Gene sequencing technology has wide application prospects in personalized medical care, cancer treatment, reproductive health and other fields.

International competition: The global gene sequencer market is dominated by companies such as Illumina and Thermo Fisher. The domestic market is gradually rising but still relies on foreign technology.

Plant DNA volume detection kit: used to detect plant genome size, ploidy level, and cell cycle.

Service content: Provides plant DNA capacity detection kits and plant flow cytometry detection, flow ploidy analysis and plant chromosome counting and other services.

Plant DNA capacity detection kit: 1000-3500 yuan per time.

Plant flow cytometry detection: 300 yuan per time.

Cell flow ploidy analysis technology: 300 yuan/time.

Plant chromosome count: RMB 2400-7900/sample.

Quick leaf cutting: Quickly process the sample to increase the concentration of nucleus in the lysate

Nuclear lysate: Achieve efficient and rapid nuclear lysis and improve the purity and yield of nucleic acid extraction.

Flow cytometry automatic injection tray: accurate limit, accurate injection.

Sample processing: collect the required samples to ensure the accuracy and reliability of the samples.

Mixing and reaction: Add the sample to the reagent and react thoroughly.

Measurement and analysis: Use the corresponding instrument to read the measurement results.

Cleaning and storage: Cleaning and disinfecting kits and utensils, storing unused reagents.

Independent research and development: breaking the passive situation where plant DNA capacity detection kits are monopolized by foreign countries.

High cost performance: The price is much lower than that of foreign kits, and the quality of data reports is better than that of foreign ones.

Technical advantages: The scientific research team masters core technology without relying on third parties.

Educational application: used for undergraduate flow cytometry teaching and experimental research, and enhance school-enterprise cooperation.

Scientific research application: Improve the efficiency of seed selection and breeding and assist scientific research institutions in research.

Corporate cooperation: Cooperate with biological enterprises to provide efficient technical services

Potential competitors: weak entry ability and high technical barriers.

Alternatives: Weak substitution capability, flow cytometry detection is at the forefront.

Consumer bargaining power: weak bargaining power, strong targeted products, and scarce suppliers.

Partners: Hubei Key Laboratory of Hubei Province Research on Special Chinese Medicine Hubei Province Medical Plant Comprehensive Utilization Engineering Technology Research Center Joint Innovation Center for the Development and Application of Endangered Rare Medicinal Plants in Wudang, Hubei Province Wuhan Fraser Gene Information Co., Ltd. BGI

Key business: Sales plant DNA capacity detection kits Provide front-end services for gene sequencing such as plant flow cytometry analysis, karyotyping analysis, chromosome counting, etc. Nanoantibody preparation business (to be developed) Cell histone extraction kits (under development)

Value Proposition: The scientific research team does not need to rely on third parties to master core technology, and the technology is not limited. Provide an overall solution for front-end plant gene sequencing services to simplify experimental steps. The kit can be applied to more than 90% of plants, breaking the barriers of foreign technology

Customer Relations: Win-win cooperation relationship Demand purchase relationship

Customer Segment: Research institutes and experimental universities Biomedical and genetic information enterprises Instrument and reagents and consumables trading companies

Core resources: Three key laboratories, six-person returnee doctoral team, eleven patented technology, four high-level papers, policy support, school platform channel access

Cost structure: R&D cost Product production cost Management cost Marketing cost

R&D and production: The company develops and produces kits and products related to gene sequencing.

Sales and channels: Products are sold through multiple channels, including platform direct sales, strategic cooperation, universities and research institutes, etc.

Provide services: In addition to selling products, the company also provides genetic testing and analysis services, etc.

Strategic cooperation: Form a strategic alliance with BGI, Wuhan Fraser and other biological enterprises to purchase front-end testing business in batches.

Customized solutions: Provide personalized solutions and follow-up sequencing data according to customer needs.

Product Pricing: Plant DNA capacity detection kit: 1000-3500 yuan/time Plant flow cytometry detection: 300 yuan/time Cell flow ploidy analysis technology: 300 yuan/time Plant chromosome count: Pricing based on the number of samples and chromosome count

New product pricing: Histone extraction kit: 600 yuan/50 times (estimated price)

Price Advantages: Compared with foreign test kits, the price is lower and the cost-effective

Direct sales: Directly connect with customers to save costs in the middle

Build and improve sales network: Expand the market through agents, raise funds quickly, and reduce financial risks

Sales Agency: Choose a powerful agent and establish long-term cooperative relationships

Media Promotion: Establish official websites, Weibo and WeChat platforms to promote publicity in professional publications and article reports

Personnel sales: Organize a high-quality marketing team to conduct door-to-door sales

Conference Sales: Participate in academic exchange meetings and seminars, conduct on-site product introduction and promotion

Public relations: Handle relationships with the public and establish a good brand image

Customer feedback mechanism: Establish a complete customer feedback channel, promptly handle customer needs and problems, questionnaire surveys and in-depth interviews and other methods Check users' real needs and suggestions for Dasi products. At the same time, real-time public opinion monitoring

Information exchange feedback platform: Build a platform for communication with customers to improve the response speed and processing efficiency of after-sales service, with the help of website messages, comments, and posts on forums. Reply.

Integration of science and education to cultivate innovative talents: The team takes the front-end service of gene sequencing as the starting point to conduct research, build a scientific research platform, provide students with scientific research opportunities, and improve students' hands-on ability and problem-solving ability.

Integration of industry and education to improve students' comprehensive abilities and help schools build first-class courses: Through project cooperation, promote the construction of first-class curriculum in the school and provide students with high-quality scientific research and teaching platforms and technologies.

Multidisciplinary cross-teams, exchanges and cooperation promote the comprehensive development of members: Create a comprehensive team with multidisciplinary intersection to promote the comprehensive development of members

Establish a Wudang Chinese Herbal Molecular Center, and deeply integrate production, education and research: Establish a scientific research team in the discipline of molecular biology to promote the deep integration of industry, academia and research.

Help popularize science, carry forward local characteristics, and enhance the pride of hometown: Through popular science propaganda and other activities, increase the public's interest in science and enhance the sense of identity in my hometown.

Leading industry technology, giving birth to new forms of industry services: Launch a series of innovative products and services to break the monopoly of foreign companies in the market.

Create characteristic plant landmarks and increase the added value of traditional Chinese medicine: Help solve problems such as germplasm and high yield of characteristic plants and promote the high-quality development of the traditional Chinese medicinal materials industry.

Cooperating manufacturers increase their income and indirectly drive employment: The company's business development drives cooperative manufacturers to increase production demand and indirectly promote employment.

Recruiting employees, recruiting agents, and technology drives future scientific research and employment: After the company is established, recruit employees, recruit agents, and drive scientific research and employment.

Market and product expansion: The team will continue to expand in both market and product aspects. The market will be based on Hubei Province and gradually expand to central China. And ultimately cover the country.

Deeply cultivate the industrial chain: in-depth research and exploration of the field of genetic testing, Apply flow cytometry to a wider range of fields.

Comprehensive solution: provide comprehensive solutions to break the monopoly position of foreign kits. Establish a new standard for plant genome size detection.

Cross-omics services: Develop cross-omics services, launch histone extraction kits, and provide high-quality teaching aids for experimental universities. Help scientific research institutions to experiment and promote the development of biological enterprises.

Promotion of technological innovation: Continuously develop new cross-species products and sample preparation and other new services, Strengthen the introduction, digestion and absorption of mature and advanced technologies at home and abroad, and carry out innovative development.

Corporate core competitiveness: Strengthen the construction of corporate core competitiveness, strictly control quality, expand business, Form a lasting and unique corporate competitive advantage.

Strategic Partnership: Strengthen cooperative relations with universities, biological enterprises, scientific research institutions, etc., and continuously improve cooperative relations. Seek more strategic partners.

Phase 1 (2024-2025): Establish a company and optimize products

Phase 2 (2025-2027): Horizontal expansion of business areas

Phase III (2027-2031): Development of In-depth Gene Sequencing

Team name: Hubei Dasi Biotechnology Co., Ltd. Established in May 2024 The team adopts a multidisciplinary cross-collaboration approach, combining expertise and technology in different fields, to promote innovation and all-round development.

Scientific Research Department: Responsible for product research and development and technological innovation. Marketing Department: Responsible for market expansion and sales. Operations Department: Responsible for brand promotion and customer service. Administrative Department: Responsible for team management and financial work

Reward system: Rewards are based on individual performance and contribution. Punishment system: Those who violate team rules and regulations are punished. Performance system: Performance assessment is conducted based on attendance, work performance, etc. Recruitment plan: recruit new members once a year and recruit new members through various channels.

Founder Team: Shi Xianming: Postgraduate student in the School of Basic Medicine, pathology and pathophysiology, and 6 years of experience in gene technology research and development. Ma Xueping: Postgraduate student at the School of Basic Medicine, Pathology and Pathophysiology, and five years of experience in medicinal plants research. Geng Lingjun: Basic Medicine - Postgraduate in Immunology, 3 years of experimental experience.

Expert technical team: You Lei: Ph.D. in biochemistry and molecular biology, presided over 3 scientific research projects. Li Chen: Director of the Hubei Cell Biology Society, won the first prize of Hubei Science and Technology Progress. Luo Xiangyin: Research direction of crop breeding, published many related papers.

Student Team: Department of Scientific Research: Ju Tingting, Zhang Mengying, Yu Tingting. Operation Department: Xu Qianqian, Jiang Shouhuan. Ministry of Administration: Zhong Luyao, Yang Zhili.

Expert guidance team: Zhang Yonghong: PhD in Biochemistry and Molecular Biology, Postdoctoral in Chemical Genetics. Xia Keke: Director of the Institute of Digital Earth, BGI Life Sciences Research Institute. Yi Xinxin: General Manager of Wuhan Fraser Gene Information Co., Ltd. Luo Ting: Senior marketing expert and SYB entrepreneurial mentor. Li Haitao: Director of the Entrepreneurship Center, lecturer of IYB and SYB.

Company registered capital: RMB 1 million

Equity Structure:

2024 (as of July):

Source of funds

Distribution ratio

Tax preferential: Enterprises are exempt from income tax for two years from the year of profit, with income tax of 7.5% from the third to eighth years Surplus reserve: from the year of profit to 10% of net profit Dividend distribution: Distribute dividend bad debt provisions at 30% of net profit: accrued at 0.5% of current receivables Employee salary: Including various benefits and insurance costs

Production cost: 2024: 677,000 yuan 2025: 1.38 million yuan 2026: 2.416 million yuan

Management and maintenance costs: 2024: 25,000 yuan 2025: 38,000 yuan 2026: 79,000 yuan

Financial expenses: 2024: 11,000 yuan 2025: 26,000 yuan 2026: 58,000 yuan

2024: Estimated revenue to reach 1 million yuan 2025: Estimated revenue to reach 2.5 million yuan 2026: The cumulative revenue is expected to exceed 5 million yuan

Return on investment of venture capital: Account for 41.81% of Das Equity. Participate in dividends since profits, and the total dividends distributed annually are 20% of net profits.

Exit mechanism:

A quick leaf cutting

Gynostem blue cleaning and drying device

A kind of cooling platform for gynostem blue

A finished product harvesting device for gynostemum blue

A Gynostem blue cultivation box

A plant flow cytonuclear dissociation solution and its application

A plant flow cytonuclear dissociation solution based on sodium cocoyl glycine and NP-40 and its application

A flow cytometer with good protection performance

A flow cytometer automatic injection tray

An integrated device for chromosomal karyotyping analysis and counting

A sample filtration device for flow cytometry