DNA Technology Tree Map

Using recombinant DNA technology and synthetic DNA, literally, any DNA sequence may be created and introduced in this mind map.

Edited at 2020-10-08 05:59:08

Fiona_

DNA Technology Tree Map

Fiona_

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Outline

DNA-Technology-Concept-Map

Explain rDNA

- Cutting DNA and pasting DNA fromdifferent sources (also known as DNAcloning)

- Involves several important tools

- Restriction enzymes

- Cloning vector

- DNA ligase

Cloning vs Gene Cloning

- Clone (Greek): described a cutting that isused to copy a plant.

- Orchid

- Roseses

- Tapioca

- A state that all organisms share exact samegenetic material.

- Cloning

- Reproduction of organism as a wholewithout sexual fertilization between maleand female gametes.

- Gene cloning

- Insertion of foreign DNA into anotherorganism.

What are required?

- Restriction endonuclease

- Also called DNA cutting enzyme orrestriction endonuclease (RE)

- The act of cutting called digestion

- Primarily in bacteria (as defense againstbacteriophage infection.

- Known as restriction site

- Other types of RE produce DNA fragmentswith double stranded ends known as bluntends

- DNA ligase

- An enzyme responsible of joining two DNAfragments

- Synthesize phosphodiester bond

- Two DNA fragments

- Between two compatible sticky ends or twoblunt ends.

- Common features between RE and DNAligase

- DNA cloning vector

- Pieces of DNA that can accept, carry andreplicate (clone) other pieces of DNA

- Important features

- Has the ability to replicate autonomously inbacterial host

- Has a site for insertion of foreign DNA

- Has a selectable antibiotic resistantmarker(s)

- Ampicilin

- Kanamycin

- Tetracyclin

- DNA plasmid

- Screening of recombinants

- Usage of selectable markers available onDNA plasmid

- Antibiotic resistant genes

- Lac Z gene (blue/white colony screening)

- Recombinant DNA tools

- Restriction endonuclease