MindMap Gallery Experimental Design
This is a mind map about Experimental Design. There are 11 main branches in the figure, namely: Isolation, culture, and activation of human CD4+T cells, Transmission of lentiviruses and knockdown of shRNA, CRISPR/Cas-9 gene editing, Analysis of flow cytometry, antibiotics, and dys, Cytokine profiling Metabolic Assays, Transcriptome Sequencing, General Metabolomics, Labeling of Glutamine and Glucose, Fluxomics in MIMOSA, Data Examinations. Each branch contains detailed descriptions of sub branches. Experimental design is crucial in scientific research, as it specifies the methods, steps, and means of the experiment, and is the foundation for ensuring the reliability and accuracy of the experimental results. For the isolation, culture, and activation experiments of human CD4+T cells, the experimental design needs to pay special attention to the source of cells, separation methods, culture conditions, and selection of activation factors. In addition, in order to achieve in-depth research on gene function, genetic engineering technology is also needed to genetically modify CD4+T cells. This includes using lentiviral vectors for gene transduction, as well as using shRNA or CRISPR/Cas-9 technology for gene knockout or knockdown. The selection and application of these technical means require detailed planning in the experimental design to ensure the smooth progress of the experiment and the acquisition of reliable results. Suitable for people interested in Experimental Design.
Edited at 2023-02-03 16:11:53