Signal generator

amplifier

Threshold adjuster

discriminator

shaper

counter

Definition of three groups of white blood cells by electrical impedance method

Small cell population (number of cells)

Intermediate cell number

large cell population

light source

sheath flow

cell suspension

light detector

Low-angle scattered light (forward scattered light)

High angle scattered light (side scattered light)

Scattered fluorescence (lateral fluorescence)

Fluorescent dyes: basic sophora yellow, polymethylene blue and propidium iodide, mainly used for nucleic acid staining

Non-fluorescent dyes: methylene blue (used for nucleic acid staining), chlorazolblack E (used for staining membrane structures of monocytes, eosinophils, neutrophils and leukocytes) and peroxidase reagent

Monochromatic light source

Detection pool

colorimetric container

light detector

Mainly used for hemoglobin determination

Improved cyanide methemoglobin hemolytic agent

Non-cyanated methemoglobin hemolytic agent

Mainly used for counting and classifying white blood cells

Mainly used for counting nucleated red blood cells

Mainly used to detect reactive lymph/plasma/hair/immature lymph/blast cells

Measure fluorescence and side-scattered light signals

Determine forward and side scattered light signals

Detects various types of white blood cells except basophils

Morphological classification for anemia

Differentiate between iron deficiency anemia (IDA) and mild dysglobinogenic anemia

Early diagnosis and efficacy observation of IDA

Parameters reflecting the heterogeneity of hemoglobin content in peripheral blood red blood cells

MDW is significantly increased in hereditary spherocytosis and can be used to screen for this disease instead of the red blood cell fragility test.

MSCV is larger than MCV in healthy people, but the opposite is true in some patients. For example, when MSCV<MCV, the sensitivity of hereditary spherocytosis is 100% and the specificity is 93.3%.

Reticulocyte percentage (RBT%), reticulocyte count

High fluorescence intensity reticulocytes (HFR), medium fluorescence intensity reticulocytes (MFR), low fluorescence intensity reticulocytes (LFR), immature reticulocyte ratio (IRF)

Reticulocyte hemoglobin content (RET-He): reflects the quality changes of reticulocytes. RET-HE is of great significance in the treatment of iron deficiency anemia.

The average amount of reticulocyte hemoglobin: can be used to evaluate the functional status of the bone marrow erythroid system. In the treatment of iron deficiency anemia, CHr is the first to increase.

Mainly include: promyelocytes, mesomyelocytes, promyelocytes

Increased IG

Includes: reactive lymphocytes, plasma cells, hair cells, immature lymphocytes, primitive cells

Increased LUA

Reference interval: 7～11fl

Increased: myelofibrosis, thrombotic disorders, prethrombotic states, splenectomy, chronic myelogenous leukemia, giant platelet syndrome

Reduced: severe infection with sepsis, hypersplenism, post-chemotherapy, aplastic anemia and megaloblastic anemia

Reference interval: 15%~17%

Reference interval: 10% ~ 30%

Increased: immune thrombocytopenia, chronic bleeding, thrombocytosis, infection

reference interval

Increased: Bone marrow hematopoietic function is good, platelet destruction in peripheral blood increases, IPF will increase

Decrease: IPF will decrease when bone marrow hematopoietic function is inhibited and platelet proliferation is poor.

1. Abnormality in the area to the left of the lymphocyte peak

2. Abnormality in the area between lymphocyte peak and single cell peak

3. Abnormality in the area between the mononuclear cell area and neutrophils

4. Abnormal area on the right side of the neutrophil peak

5.Multiple parts alarm

Cold coagulated blood specimen

Lipemia specimen

Red blood cell thread specimen

Hemolyzed specimen

Red blood cells are sensitive to the EDTA anticoagulant

nucleated red blood cells

Giant platelet specimen

Samples that are difficult to hemolyse or have poor hemolysis

microcytic anemia specimen

Platelet aggregation or platelet satellite phenomenon

cell debris

Plasmodium and Howell-Jolly bodies falsely increase reticulocytes