Inhibitors compete with substrates for the enzyme's active site

Inhibitors and substrates are structurally similar

Inhibitory effects can be reversed by increasing substrate concentration

Inhibitors temporarily occupy the active site

Prevent the binding of substrate and enzyme

Prevents normal catalytic reactions

Inhibitors do not permanently bind to the enzyme

Inhibitory effects can be reduced by increasing substrate concentration

Vmax remains unchanged, Km increases

Shown as an increase in Michaelis-Menten constant Km

Organophosphorus pesticides as competitive inhibitors

Active site of enzyme that competes with acetylcholine

Malonic acid and succinic acid have similar structures

Competitively inhibits the oxidation reaction of succinic acid

Designing drugs using the principle of competitive inhibition

used to treat certain diseases

Development of competitive inhibitors as pesticides

Control pests by inhibiting their specific enzymes

Controlling enzymatic reactions in industrial processes

Adjust product yield by adding competitive inhibitors

Prevent substrate from entering the active site

Change the shape or charge of the active site

Through the accumulation of NADH

via phosphorylase kinase

Regulatory sites for specific enzymes

Explore regulatory mechanisms beyond the active site of enzymes

Inhibitors compete with the substrate for the same active site

Inhibition can be overcome by increasing substrate concentration

Inhibitors and substrates do not compete for the same active site

Inhibition cannot be overcome by increasing substrate concentration

Inhibitor binds to enzyme substrate complex

leading to reduced enzyme activity

In competitive inhibition, the inhibitor competes with the substrate for the same binding site.

Usually a region distinct from the active site

Makes the formation of enzyme-substrate complexes difficult

Does not affect Vmax (maximum reaction rate)

When the substrate concentration is high enough, the inhibitory effect is weakened

Substrate concentration-independent properties are key to its definition

For example, the inhibitory effect of sulfa drugs on bacteria

For example, feedback inhibition of certain metabolic pathways

Development of anticompetitive inhibitors against specific enzymes

Regulation of enzymatic reactions by anticompetitive inhibition

Analyzing the type of inhibition by measuring reaction rates

Predict the binding mode of inhibitors to enzymes

Determine the three-dimensional structure of inhibitor-enzyme complexes